RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site
نویسندگان
چکیده
منابع مشابه
Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.
A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo site-specific translocation of their component segments or inversion of their ori...
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Existing methods for site-directed plasmid mutagenesis are restrained by the small spectrum of modifications that can be introduced by mutagenic primers and the amplicon size limitations of in vitro DNA synthesis. As demonstrated here, the combined use of Red/ET recombination and unique restriction site elimination enables extensive manipulation regardless of plasmid size and DNA sequence eleme...
متن کاملComparison of Single- site and Multi-site Based Calibrations of SWAT in Taleghan Watershed, Iran
Calibration of model is critical for hydrologic modeling of large watersheds in a mountain watershed. In this study Soil and Water Assessment Tool (SWAT) used to comparison a single-site calibration procedure that employed streamflow measurement at outlet of watershed to a multi-site calibration method that used streamflow measurements at three stations (Galinak, Joestan and Dehdar). Results sh...
متن کاملEcoRI restriction endonuclease cleavage site map of bacteriophage P22DNA.
‘l’l~e SWYW EcoKI restriction endonuclease cleavage sites in bacteriophage P%d DNA have been mapped. The cleavage site map of circularly permut.ed P22 linear DNA is a circle. The positions of EcoRI sites in the early region of the P22 genomc were determined by comparing products of EcoRI digestion of maturtx liaear P22 chromosomes with the EcoRI cleavage fragments of DNA of three XimmP22 hybrid...
متن کاملThe cleavage site of the restriction endonuclease Ava II.
We have determined that the type II restriction enzyme Ava II, isolated from Anabaena variabilis, recognizes and cuts the sequence (formula: see article). The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava I.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.21.5831